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Journal:
Article Title: Identification of Arabidopsis Ethylene-Responsive Element Binding Factors with Distinct Induction Kinetics after Pathogen Infection
doi: 10.1104/pp.010862
Figure Lengend Snippet: RNA expression of the Arabidopsis ERF genes in response to pathogen infection and SA treatment in the pad4-1 mutant. A, Infection of WT and pad4-1 with P. syringae. Arabidopsis leaves from 4-week-old plants were infiltrated with a mock solution (m) or with the same solution containing approximately 107cfu mL−1 of the avirulent pathogen Pst DC3000(avrRpt2) (av) or approximately 107cfu mL−1 of the virulent pathogen Pst DC3000 (vir). RNAs isolated from leaves at the indicated time points were subjected to RT-PCR. B, Treatment of WT and pad4-1 with SA. Four-week-old Arabidopsis plants were sprayed with 0.1% (v/v) ethanol (−) or 1 mm SA or were not treated (nt). RNAs isolated from leaves after 6 h of treatment were analyzed by RT-PCR.
Article Snippet: We infiltrated leaves of Arabidopsis plants containing the RPS2 resistance gene with either a mock solution or a suspension containing the
Techniques: RNA Expression, Infection, Mutagenesis, Isolation, Reverse Transcription Polymerase Chain Reaction